benoit at nibh.go.jp
Wed Jan 24 05:17:23 EST 1996
I am actually trying to perform 2D gels with S. cerevisiae crude extracts.
But the resolution of the spots is not that good and I would like to
stretch the region of the gel comprised between pH 6 and 8, and molecular
weight between 15000 and 50000.
I use NaOH 100 mM and phosphoric acid 10 mM.
The ampholyte concentrations are :
1.6% ampholyte 5/7
0.4% ampholyte 3/10 in both first dimension gel and first dimension sample
0.8% ampholyte 5/7
0.2% ampholyte 3/10 in sample overlay buffer.
How can I modify these concentrations to enlarge the interesting zone ?
Thanking you in advance.
B. VIARD, Postdoctoral Researcher
National Institute of Bioscience and Human-Technology
Biochemical Engineering Laboratory
1-1, Higashi, Tsukuba
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