Help: Expression w/ PHO5 promoter
JPhillips at Genencor.com
Thu Jan 25 21:06:46 EST 1996
I would like to do some yeast expression experiments using the
PHO5 promoter. In addition, I would like to try some of these
experiments with a strain with a pho4 temperature sensative mutation.
I have found a paper that describes a strain designated 29B5
(pho4ts, pho80) (R.A. Kramer, et al. (1984), "Regulated expression of
a human interferon gene in yeast: Control by phosphate concentration
or temperature", Proc. Natl. Acad. Sci. USA, Vol. 81, pp 367-370).
Unfortunately, the authors of this paper were at the Roche Research
Center, which I have been led to believe is now defunct, and I have
been unsuccessful in locating the whereabouts of the authors. I have
also been unsuccessful in locating such a strain in any of the databases
If any kind soul can give me information that can lead me to either
a strain with a pho4ts mutation (and maybe with a pho80 mutation as
well), or the authors of the paper above I would be ever so grateful.
JPhillips at genencor.com
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