Homologous Recombination in Yeast

David Roof Roof at mail.med.upenn.edu
Fri Jan 26 15:51:27 EST 1996


In article <4e5bnc$637 at mserv1.dl.ac.uk>, "Miguel A. RODRIGUEZ-GABRIEL"
<MARODRIGUEZ at mvax.cbm.uam.es> wrote:

>     I have one question (maybe more!) concerning homologous recombination in 
> Saccharomyces cerevisiae. My main questions are:
> 
>     1. Minimum homologous fragment size needed for homologous recombination.
>     2. Percentage of homology needed for this homologous recombination.
> 
>     If you know about some reports in these subjects, please post your
answers 
> or contact me directly. I will post a summary of the answers I'll receive.
> 
>     Thanks in advance,
>     Miguel A. RODRIGUEZ-GABRIEL

Just 45 bp at each end of a selectable marker is enough to direct
homologous recombination, provided the original locus for the selectable
marker has been deleted.  See Baudin et al, NAR 21:3329-3330, 1993.  This
allows direct gene deletion using a PCR fragment.
David Roof



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