y2h plasmids

[Jeffrey David Bray]

Hi everyone,

The common plasmids used for y2h fuse the gene of interest to the
carboxy terminus of either GAD or GDD.  Does a vector exists that will
allow you to fuse your favorite gene to the amino terminus, i.e. has a
polylinker before the GAL4 piece?  I would be very grateful if anyone
could help me out on this one.  Thanks


Jeff Bray
Bilogical Sciences
Carnegie Mellon University