HB101 and Leu plasmids

[John Hogenesch]

Help,
	We are trying to rescue Leu2 based plasmids from yeast (also 
containing TRP based plasmids).  It doesn't look like the M9 media 
supplemented with proline, thiamine, and amp is keeping the TRP based 
plasmids from recueing.  As a control, I transformed pGEMt, and it 
survived on these plates as well.  Has anyone else out there had this 
problem?  I have tried another aliquot of HB101 cells and am ordering 
more from promega.

Thanks in advance,

John Hogenesch
MPBC/Northwestern University