ever seen GFP in S cerevisiae ?

Guillaume Stahl stahl at infobiogen.fr
Wed Jun 5 08:32:48 EST 1996


Hi GFPyeastNetters,

I've integrated recently a fusion protein with wt GFP en Cterm in the 
cerevisiae genome. After YPD culture (+/- 1 OD), directly under scope, 
using fluoresceine filters, I've not been able to see anything (except 
some cells, strange shape, greennish/yellowish). Same result with same 
culture O.N. in the fridge, and same result with... negative control.

My question is simple : what are the condition (media, OD, wash, fridge, 
filters,...) you used if you ever saw GFP in yeast. Do you think a 
single copy of 'medium expressed' GFP is sufficient. Should I try it in 
a FACS ? Do you have good conditions/suggestions for the last point ?

Thanks in advance

Guillaume Stahl, Orsay, France



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