complementation of a budding yeast mutation with a fission yeast gene

Andy Foster A.J.Foster at exeter.ac.uk
Fri Jun 7 06:45:25 EST 1996


I am working with fission yeast and wish to attempt the 
complementation of a budding yeast mutation with a fission yeast 
gene. I wonder if co transformation with a fission yeast and a budding 
yeast vector would be likely to give rise to rearrangements such that the 
pombe gene goes into the budding yeast vector. This works well in 
fission yeast but I dont know if this is true of budding yeast.
Can anyone help?
Andy Foster (Exeter University)
(I mailed this message under someone elses name before!)



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