AD-fusion vectors

John Hogenesch john at pc029.pharm.nwu.edu
Wed Jun 12 14:51:27 EST 1996


Yeast and 2-hybrid people,
	Does anybody out there have a LEU-based GAL or VP16 AD fusion 
vector where the AD is c-terminal or know where I can request one?  I 
tried using a GALAD-fusion vectors (pGAD424) and for some reason our 
proteins don't interact with the AD at the n-terminus.  I know that the 
problem is contextual because I've cloned the TAD onto the c-terminus 
and they work great.  Thanks for any help,

John Hogenesch
MPBC/Northwestern University



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