help

Isidro Contreras Morales a73055 at UACHIH.UACHNET.MX
Fri Jun 14 15:19:01 EST 1996


 Hi everyone.

    I need to find protocols for Northern and ribosomal RNA electrophoresis 
courrently working with yeast and I have tecnicals troubles.

   I have run electrophoresis 5, 10, 15 ug of rRNA  was extracted with:
a) acidified phenol, heating to 65§ C.
b) phenol-clorophormo- isoamilic and glass beads(bortex).
   and cuantified by OD at 260 nm.

   I heve also stain the agarose gels with etidium bromide and I have not 
seen the bands 25S and 18S rRNA.
   All my glassware has been trated with DEPC.


   I shall be gratefull for any help.
 

* Isidro Contreras Morales           * Email: a73055 at uachih.uachnet.mx
* Division de Estudioos de Posgrado  
* Facultad de Ciencias Quimicas      * Tel. (14) 13-18-28 Ext.117    
* Universidad Autonoma de Chihuahua  * Fax. (14) 14-44-92
* Apartado Postal 1542-C



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