genome info ??

KARL KLEINE <KLEINE at MIPS.EMBNET.ORG> KLEINE at MIPS16.dnet.mips.biochem.mpg.de
Tue Jun 18 03:18:19 EST 1996



Dear Netters,

in reply to a more or less simple question (everybody should be able to
calculate himself) I was curious to see the numbers which were requested.


Glen (gshearer at whale.st.usm.edu) asked for:


1. GC content of the yeast nuclear genome
   The genomic sequences currently available at MIPS contain 12,067,280 nt
   and the GC content of this portion is 38.30 % .
   Assumed that there are 120 repeats of rDNA with 9138 nt each the GC content
   changes to 38.751 % because the rDNA repeats have a GC % of 43.751 .


2. amount of repetitive sequences
   The rDNA repeats are already mentioned and there might be some more
   including  the Y' sequences, the CUP repeats, the ENA repeats.
   My personal estimation therefore is (again assuming 120 rDNA repeats):
     120 *  9138 (rDNA)
   + ~30 * ~6000 (Y')
   being a total of 1,276,560 nt that is close to 10% of the total genome!
   (The CUP and ENA repeats don't count too much to the total genome)

   This does not cover what is called redundancy in the yeast genome!!!
   (eg. pairs of ribosomal proteins, families of sugar transporters, etc.)

3. fg DNA/nucleus

   assuming 1bp = 660 g/Mol 
   then you end up with 
   13163840 bp * 660 g = 8,6881 Gg/Mol yeast nuclei

   which is 14.425 fg/nucleus (haploid) (1 Mol = 6.023E+23)


Have fun!

 Karl 

^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
*                                       |             E-mail:                *
*           Dr. Karl KLEINE             |      kleine at mips.embnet.org or     *
*                                       | kleine at ehpmic.mips.biochem.mpg.de  *
*                 MIPS                  |              Tel:                  *
* am Max-Planck-Institut fuer Biochemie |        +49 89 8578 2659            *
*          Am Klopferspitz 18a          |              Fax:                  *
*          D-82152 Martinsried          |        +49 89 8578 2655 or         *
*                Germany                |   +49 89 8578 3532 ("Yeast-Fax")   *
*~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~*
* Internet connectivity to MIPS  - WWW-home: http://WWW.MIPS.BIOCHEM.MPG.DE  *
*                                - FTP:      FTP.MIPS.EMBNET.ORG             *
*                                - Email:    username at MIPS.EMBNET.ORG        *
*----------------------------------------------------------------------------*




More information about the Yeast mailing list