Northerns...again

Josee Ash ASH at BIOTECH.LAN.NRC.CA
Wed Jun 19 09:50:34 EST 1996


Bonjour Marguerite!

I tried to contact you by e-mail, but it didn't work.  My suggestion is
that you should avoid blotting your membrane on whatman paper.  I
usually put my membrane directly in pre-hyb. solution after stripping. 
Another observation we made is when we do Southern blot with the dig
non-radioactive detection system, blotting a wet membrane on whatman
paper always gives high  background.  I hope this will help you.

Josee Ash
CNRC-IRB
Montreal
ash at biotech.lan.nrc.ca



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