Yeast dissection/fiber optic needle

Bob Johnson bjohnson at SIEGFRIED.UTMB.EDU
Fri Mar 15 15:54:49 EST 1996


Well, since you bought the microscope, I guess you have to use it, as 
dreadful as that may be.  We've searched to find needles specifically 
designed for dissections but haven't found anything; everything is 
microinjection based.  We know of using fiber optic needles, you just 
glue it onto the end of a glass rod, but for some reason we have always 
used, and still use, glass rods. Maybe it's just that why change 
something that works.  We use 3mm outside diameter pyrex (glass) rod 
from Corning. We pull the needles over an open flame, pulling the needle 
at a 90 degree angle to the length of the glass rod so that it points 
upward when dissecting.  It takes practice and some foul language, but 
we get good needles, and we gaurd them with our lives!  Anyway I can't 
tell you where to get fiber optic, but thought you might want another 
idea about needles.  If you need some more info about this method,feel 
free to write back.
  
Bob Johnson



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