REQUEST: S. pombe lab strain, expression vector

Arle Kruckeberg kruckeba at biovx1.biology.ucla.edu
Sat Mar 16 18:46:42 EST 1996


Hello out there--
Can anyone provide me with a good expression vector for Schiz. pombe?
The usual desirable features (ars1/stb for stability, a good
auxotrophic marker like leu1 or ura4), and a well-regulated promoter
(such as the nmt1 promoter or glucocorticoid response element system).
Also a good, standard strain that is auxotrophic for the marker on the
vector, and is otherwise more or less wildtype? And, please, a map of
the vector and/or appropriate references? If you can help, please drop
me a line.
Gratefully,

Arle
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a.k.a. Dr. Arthur L. Kruckeberg
 Department of Biological Chemistry
UCLA School of Medicine
Los Angeles CA 90024
(310) 825-8363     FAX (310) 206-5272
e-mail kruckeba at biovx1.biology.ucla.edu
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