LexA or GAL4?

[Tohru Ikegami]

Hi yeasties,

	I have a few questions about two-hybrid system in Saccharomyses 
cerevisiae.  
	I'd like to find Baker's yeast proteins that interact with our yeast 
protein fused LexA or GAL4(1-147) as the "bait" for yeast two-hybrid 
approach.  

	Is there any advantages or disadvantages in each case of LexA or GAL4 
using for two-hybrid system in general?  

	How about in the case of using hi-expression vector ex. 
pAS2(CLONTECH)? 

	Thanks a lot for any advice. 

Tohru Ikegami
Mol. Biol. Inst. Med.Sci.
Univ. Tokyo, JAPAN
faryeast at ims.u-tokyo.ac.jp