IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

LexA or GAL4?

Tohru Ikegami faryeast at hgc.ims.u-tokup.ac.jp
Mon Mar 18 03:01:49 EST 1996

Hi yeasties,

	I have a few questions about two-hybrid system in Saccharomyses 
	I'd like to find Baker's yeast proteins that interact with our yeast 
protein fused LexA or GAL4(1-147) as the "bait" for yeast two-hybrid 

	Is there any advantages or disadvantages in each case of LexA or GAL4 
using for two-hybrid system in general?  

	How about in the case of using hi-expression vector ex. 

	Thanks a lot for any advice. 

Tohru Ikegami
Mol. Biol. Inst. Med.Sci.
Univ. Tokyo, JAPAN
faryeast at ims.u-tokyo.ac.jp

More information about the Yeast mailing list

Send comments to us at biosci-help [At] net.bio.net