expression vector

T.SUZUKI TSUZUKI at CCIT.ARIZONA.EDU
Wed Mar 20 13:22:51 EST 1996


Dear All,
I am a graduate student trying to express a plant small heat shock protein
in yeast.  I am looking for an expression vector with the following
properties:
	1.  tryptophan marker
	2.  yeast terminator/poly A site
	3.  Good multiple cloning site for my gene (EcoRI=ideal)
	4.  Nice strong promoter
I've checked most of the major companies (eg. Invitrogen, Stragene...) and
the ATCC.  For a second experiment a HIS4 or URA3 marker would also work
(this would be similar to the Invitrogen pYES2, which can be purchased
for $220 if I REALLY need it.  Has anyone tried pYES2?  What do you think?).
Expression level is the bottom line, does higher copy number generally
give better results, all other things being equal?
Thanks for any information  you can share!
Teri Suzuki
Dept. Biochem., U of A
Tsuzuki at ccit.arizona.edu



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