HA- or FLAG- tag for yeast

[Preston Garrison]

In Article <mcc9-0905961222320001 at 128.84.203.78>, mcc9 at cornell.edu (Maria C.
Costanzo) wrote:
>In article <9605082026.AA32222 at mis.finchcms.edu>, muellerd at finchcms.edu
>("Dr. David Mueller") wrote:
>
>> Hi yeasters:
>> 
>> I would like to do immunolocalization studies in yeast S. cerevisiae.  Do 
>> the commercial antibodies against HA or FLAG epitopes cross react with 
>> anything in yeast?....

>In my experience, the anti-HA antibody cross-reacts strongly with a
>protein of about 43 Kd.
>
>Maria
>
I too have seen a strongly reacting band with the anti-HA monoclonal, 
although I don't remember the exact size. (Does this mean that yeast 
can get the flu?)

Preston Garrison
garrisonp at uthscsa.edu
Biochem. Dept.
Univ of Texas Health Sci Ctr
San Antonio, Tx 78284-7760 USA
210-567-3702