(no subject)

Kathy Baynton baynton at esbs.u-strasbg.fr
Wed Nov 6 09:48:17 EST 1996


I am currently doing biochemical analyses of plasmids transformed into 
yeast and I double check these results by isolating these plasmids and 
retransforming them into E. coli.  I was wondering if anyone had a 
"feeling" as to whether the plasmids recovered from yeast would be 
"faithfully" replicated in E. coli.  These vectors are pUC-based with a 
yeast 2micron and TRP1 gene.  I have heard conflicting views, and was 
wondering if anyone may have some insight into this.  ANY comments, 
etc., are appreciated!!

Kathy Baynton





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