Plasmid recovery from yeast

lobreaux at ensam.inra.fr lobreaux at ensam.inra.fr
Thu Nov 7 04:00:51 EST 1996


Dear netters
I am trying to complement a yeast mutant with a plant cDNA library,
constructed in lambda PG15. After screening, I have many putative
clones and I am trying to extract the plasmid and tranform E. Coli.
So far,  I haven't been able to obtain colonies with my different
extracts.
The mutant I use doesn't grow very well in YNB.
I would appreciate any suggestion to try to solve this problem.
Thank you by advance

Stephane LOBREAUX
lobreaux at ensam.inra.fr

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