ade3 dependent Histidine auxotrophy
walberg at simmons.swmed.edu
Wed Nov 13 14:44:30 EST 1996
J. David Stepp wrote:
> Daniel Schott mentioned in passing that ade3 strains cannot synthesize
> histidine. We've seen His auxotrophy in ade3 strains but were unaware that it
> was directly related. Can others confirm this phenomenon? Thanks.
> Dave Stepp
Yes, ade3 strains are definitely histidine auxotrophs.
Ade3 is involved in one carbon metabolism, and its function
is required for synthesis of both adenine and histidine.
A great place to read about this is in the Cold Spring Harbor
S.cerevisiae books from about 1981 or 1982.
If you need to use your his3 marker in a selection,
just put an ADE3 plasmid in first. It works just fine then.
Mark Walberg walberg at simmons.swmed.edu
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