re. size of fusion protein in two-hybrid system

Paul Andrew Smith PAULS at SRV0.BIO.ED.AC.UK
Fri Nov 15 07:25:48 EST 1996

Dear David,
I have performed a two-hybrid screen with a bait protein just over 
100 kDa in size (not including the Gal4 DNA binding domain). The 
screen was successful  in that it identified an interacting protein which 
had previously been suggested to interact with my protein of interest  by
genetic experiments.

Paul Smith
University of Edinburgh

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