Better B-gal substrate???

[Brett Burkholder]

Daniel Harkness wrote:
> 
> Does anyone have any information regarding a substrate for b-gal 
> overlays that might more sensitive that X-Gal?  I remember hearing 
> about a substrate that when cleaved, gave a "red" color.  Any 
> references or suggestions?
> 
> Thanks,
> Dan Harkness
> harkness at bio.bu.edu


In our liquid enzyme assay we use chlorophenol 
red-Beta-D-galactopyranoside (CPRG) from Boehringer Mannheim. It produces 
a "Red" color when cleave by Beta-galactosidase.  I dont think it would 
work for filter lifts,as the chromophore is soluble in water, but it 
works great for the liquid enzyme assay.

Brett Burkholder
Genetics and Molecular Medicine
Emory University