Better B-gal substrate???
burkhold at gmm.gen.emory.edu
Tue Nov 19 11:42:35 EST 1996
Daniel Harkness wrote:
> Does anyone have any information regarding a substrate for b-gal
> overlays that might more sensitive that X-Gal? I remember hearing
> about a substrate that when cleaved, gave a "red" color. Any
> references or suggestions?
> Dan Harkness
> harkness at bio.bu.edu
In our liquid enzyme assay we use chlorophenol
red-Beta-D-galactopyranoside (CPRG) from Boehringer Mannheim. It produces
a "Red" color when cleave by Beta-galactosidase. I dont think it would
work for filter lifts,as the chromophore is soluble in water, but it
works great for the liquid enzyme assay.
Genetics and Molecular Medicine
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