PCR using Yeast colonies-info wanted

Joerg Schenk jschenk at mdc-berlin.de
Mon Nov 25 03:30:38 EST 1996


We amplified successfully sequences of plasmids from yeast colonies. Have
a look at BioTechniques 20: 812-816 for details.

Good Luck

Joerg Schenk


On Fri, 22 Nov 1996, John Stebbins wrote:

> I am going to try to pcr a fragment out of yeast genomic DNA.  I seem to
> remember a post that descibed adding some yeast straight from a plate into
> the reaction and letting the 95degrees lyse it.  Anyone know the details of
> this and how well it works?




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