GST fusions

David Roof Roof at mail.med.upenn.edu
Wed Oct 2 17:10:18 EST 1996


In article <324B08BF.6F11 at BIOSCI.MBP.MISSOURI.EDU>,
NOTHWEHR at BIOSCI.MBP.MISSOURI.EDU wrote:

> N-GST-proteinX-C fusion proteins can be easily purified based on the 
> affinity of GST to glutathione-agarose.  Does anyone know if it is 
> possible to construct fusions to the opposite end of GST (i.e. 
> N-proteinX-GST-C) and still maintain proper folding of the GST domain?  
> If so, does anyone know if vectors have been constructed which make 
> this type of fusion convenient to construct and where I could obtain 
> such vectors?  Thanks.

We've made N-proteinX-GST-C fusion proteins and they bind glutathione
agarose just fine.  Our constructs were made by PCR.  I have not noticed
any commercial vectors set up to do this.
--David



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