Protocol for GAL1 promoter induction.

Guy Oshiro Guy.Oshiro at UCHSC.edu
Wed Oct 2 13:04:46 EST 1996


another thing to look at is to make sure that your strain is not Gal1
minus.  These strains can not use Galactose as a carbon source; so
these need to be supplemented with Raffinose as a carbon source.  But I
doubt that this is the case in your situation because it would have
been pretty obvious if your culture did not grow.

I concur with the previous post of harvesting after a short induction
time 2-5 hrs versus o/n.


Guy Oshiro
EMAIL: Guy.Oshiro at UCHSC.edu

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