okadaic acid sensitivity

Francisco G da Nobrega ib - bio 7588 fgdnobre at usp.br
Wed Oct 2 08:00:53 EST 1996


Dear Leibowitz:

The references below bear on okadaic acid and also sphingolipids and 
ceramide. Fisbein tests okadaic on yeast.

Cuvillier, O. et al. (1996). Suppression of ceramide-mediated cell death 
by 
sphingosin-1-phosphate. Nature, 381(27 jun), 800-803. 

Fishbein, J. D., Dobrowski, R. T., Bielawska, A., Garret, S., & Hannun, 
Y. 
A. (1993). Ceramide-mediated growth inhibition and CAPP are conserved in 
Saccharomyces cerevisiae. J. Biol. Chem., 268(5 may), 9255-9261. 

Morell, P., & Radin, N. S. (1970). Specificity in ceramide biosynthesis 
from 
long chain bases and various fatty acyl coenzyme A's by brain 
microsomes. J. Biol. Chem., 245(25 jan), 342-350. 

Nickels, J. T., & Broach, J. R. (1996, February 15). A ceramide-activated 
protein phosphatase mediates ceramide-induced G(1) arrest of 
Saccharomyces cerevisiae. Genes & Development, 10(4), 382-394. 

Singh, I., & Kishimoto, Y. (1978). A novel synthesis of ceramide from 
lignoceric acid and sphingosine by rat barin preparation; the amide 
formation requires a pyridine nucleotide. B.B.R.C, 82(29 jun), 1287-
1293. 

Thorpe, S. R., & Sweeley, C. C. (1967). Chemistry and metabolism of 
sphingolipids. On the biosynthesis of phytosphingosine by yeast. 
Biochemistry, 6(mar), 887-898? 

Sincerely

F.G. Nobrega
Universidade de Sao Paulo
Departamento de Microbiologia - ICB II / USP
Av. Prof. Lineu Prestes, 1374
05508-900   Sao Paulo  -  SP  - Brasil
Voice: (011) 818-7588    Fax: 55-11-818-7354    E-mail:  fgdnobre at usp.br

On 1 Oct 1996 LEIBOWITZ at ocelot.rutgers.edu wrote:

> Dear yeasters,
> 
> The phosphatase inhibitor okadaic acid inhibits some phosphatases extracted from
> from yeast, as it does similar enzymes from "higher" organisms.  However, I 
> have been unable to find any information on whether okadaic acid will act on 
> the enzymes in intact  yeast cells, as it does in cultured vertebrate cells.  
> I would be grateful to anyone who can provide info. about the in vivo effect 
> of this compound on yeast.
> 
> Sincerely,
> Mike Leibowitz
> UMDNJ-RW Johnson Medical School
> 
> 



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