Immunofluorescence Backround

Fri Oct 4 18:17:51 EST 1996

To all, 
	I am doing indirect immunofluorescence on S.cerevisiae YPH499. I am 
having a problem with high backround i.e. the preimmune control lights 
up like a Christmas tree. We are about to affinity purify the Ab. What 
fixatives or levels of blocking has anyone had success in reducing 
backround? Our Ab looks pretty good on a western. Thanks in advance.

Geoff Meacham
UAB Dept. of Cell Biology

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