Jose Ribamar dos Santos Ferreira Junior
zeribajr at usp.br
Tue Oct 8 14:46:56 EST 1996
On 3 Oct 1996, Dr. Michael Ziman wrote:
> I was wondering, why one has to use minimal plates for 5-FOA. Can't I just
> use YPD?
> Any comments would be appreciated!
The use of 5-FOA is to select against the functional URA3 gene.
If you have a strain with this gene functional, naturally, it will grow i
YPD plates. You must to give 5-FOA - an analog of pyrimidine - for this
type of "negative seklection".
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