5-FOA

Jose Ribamar dos Santos Ferreira Junior zeribajr at usp.br
Tue Oct 8 14:46:56 EST 1996


On 3 Oct 1996, Dr. Michael Ziman wrote:

> Hi!
> 
> I was wondering, why one has to use minimal plates for 5-FOA. Can't I just
> use YPD?
> 
>       Any comments would be appreciated!
> 
>                Anne
> 

	Dear Anne,

	The use of 5-FOA is to select against the functional URA3 gene. 
If you have a strain with this gene functional, naturally, it will grow i 
YPD plates. You must to give 5-FOA  -  an analog of pyrimidine - for this 
type of "negative seklection".
			Best Regards.
						
						Riba




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