FOA

Brian Wickes wickes at cgl.ucsf.edu
Thu Oct 10 12:53:53 EST 1996


How's this for an answer;  The reason that YNB and not YPD is preferred 
is that you want the cells to quickly suck up the FOA.  Although YNB 
does have plenty of nitrogen, there is no question that cells grow 
better on YPD and therefore may not take up the FOA as rapidly.  
Therefore you need more FOA which in turn requires you to ultimately 
spend more money!  There was a paper put out a few years ago showing 
that, in fact, if you reduce the amount of added uracil (which allows 
mutants which will be resistant to FOA to survive) you can use less FOA.  
I have found that if you do that as well as reduce the level of dextrose 
you get even more mutants--most likely due to starvation which causes 
the cells to really crank up the permease activity.

Brian Wickes





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