pRS317 Sequence

Stephen Bell spbell at mit.edu
Wed Oct 16 18:32:39 EST 1996


Netters, 

	   Thanks for the responses concerning the gapped-plasmid 
PCR co-transformation experiments.  I have a new question.  
Does anyone know where the sequence of the pRS317 Hieter 
plasmid can be downloaded from?  It does not seem to be in 
NCBI that I can find.  If not, does anyone know if it is the 
RI to HIII fragment of LYS2 that is blunted into the same 
site as the URA3 etc. genes in other Hieter vectors?  Thanks 
again for your help.  

Sincerely, 

Steve Bell



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