tagging mutagenesis in yeast??

Mark Walberg walberg at simmons.swmed.edu
Thu Oct 24 12:57:11 EST 1996


Eckhard Boles wrote:
> 
> Hi,
> 
> I would like to perform a random mutagenesis with a specific S.
> cerevisiae mutant strain selecting for a positive phenotype which enables
> me to identify the corresponding gene. Can anyone provide me with
> information about tagging mutagenesis systems in yeast, e.g. inducible Ty
> transposable elements on a plasmid, which after creating the
> insertional mutation allow to identify the mutated gene?
> 
> Thanks a lot for your help.
> EBo

Read the paper from Mike Snyder's lab:
Burns et al. 1994. G&D 8, 1087



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