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tagging mutagenesis in yeast??

Eckhard Boles boles at uni-duesseldorf.de
Thu Oct 24 11:37:31 EST 1996


I would like to perform a random mutagenesis with a specific S. 
cerevisiae mutant strain selecting for a positive phenotype which enables 
me to identify the corresponding gene. Can anyone provide me with 
information about tagging mutagenesis systems in yeast, e.g. inducible Ty 
transposable elements on a plasmid, which after creating the 
insertional mutation allow to identify the mutated gene?

Thanks a lot for your help.

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