tagging mutagenesis in yeast??
Fri Oct 25 14:43:57 EST 1996
In article <54qst9$mqr at nntp3.u.washington.edu>, David Friedman <dfried@> wrote:
>In article <326FADF7.270B at simmons.swmed.edu>,
>Mark Walberg <walberg at simmons.swmed.edu> wrote:
>>Eckhard Boles wrote:
>>> I would like to perform a random mutagenesis with a specific S.
>>> cerevisiae mutant strain selecting for a positive phenotype which enables
>>> me to identify the corresponding gene. Can anyone provide me with
>>> information about tagging mutagenesis systems in yeast, e.g. inducible Ty
>>> transposable elements on a plasmid, which after creating the
>>> insertional mutation allow to identify the mutated gene?
>>> Thanks a lot for your help.
>>Read the paper from Mike Snyder's lab:
>>Burns et al. 1994. G&D 8, 1087
>Dept. of Biochemistry, Box 357350 "I am not a number,
>U of Washington, Seattle WA 98195 I am a Friedman!"
try Chun et al in the Jan 1996 Genetics.
Dept. of Biochemistry, Box 357350 "I am not a number,
U of Washington, Seattle WA 98195 I am a Friedman!"
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