tagging mutagenesis in yeast??

David Friedman dfried at
Fri Oct 25 14:43:57 EST 1996


In article <54qst9$mqr at nntp3.u.washington.edu>, David Friedman <dfried@> wrote:
>In article <326FADF7.270B at simmons.swmed.edu>,
>Mark Walberg  <walberg at simmons.swmed.edu> wrote:
>>Eckhard Boles wrote:
>>> 
>>> Hi,
>>> 
>>> I would like to perform a random mutagenesis with a specific S.
>>> cerevisiae mutant strain selecting for a positive phenotype which enables
>>> me to identify the corresponding gene. Can anyone provide me with
>>> information about tagging mutagenesis systems in yeast, e.g. inducible Ty
>>> transposable elements on a plasmid, which after creating the
>>> insertional mutation allow to identify the mutated gene?
>>> 
>>> Thanks a lot for your help.
>>> EBo
>>
>>Read the paper from Mike Snyder's lab:
>>Burns et al. 1994. G&D 8, 1087
>
>
>-- 
>_______________________________________________________________________________
>David Friedman                          
>Dept. of Biochemistry, Box 357350             "I am not a number,
>U of Washington, Seattle WA 98195                  I am a Friedman!"

try Chun et al in the Jan 1996 Genetics.
-- 
_______________________________________________________________________________
David Friedman                          
Dept. of Biochemistry, Box 357350             "I am not a number,
U of Washington, Seattle WA 98195                  I am a Friedman!"



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