tagging mutagenesis in yeast??

David Friedman dfried at
Fri Oct 25 12:20:41 EST 1996


In article <326FADF7.270B at simmons.swmed.edu>,
Mark Walberg  <walberg at simmons.swmed.edu> wrote:
>Eckhard Boles wrote:
>> 
>> Hi,
>> 
>> I would like to perform a random mutagenesis with a specific S.
>> cerevisiae mutant strain selecting for a positive phenotype which enables
>> me to identify the corresponding gene. Can anyone provide me with
>> information about tagging mutagenesis systems in yeast, e.g. inducible Ty
>> transposable elements on a plasmid, which after creating the
>> insertional mutation allow to identify the mutated gene?
>> 
>> Thanks a lot for your help.
>> EBo
>
>Read the paper from Mike Snyder's lab:
>Burns et al. 1994. G&D 8, 1087


-- 
_______________________________________________________________________________
David Friedman                          
Dept. of Biochemistry, Box 357350             "I am not a number,
U of Washington, Seattle WA 98195                  I am a Friedman!"



More information about the Yeast mailing list