brefeldinA

x at cornell.edu x at cornell.edu
Sun Oct 27 12:00:54 EST 1996


In article <aspang-2210962013020001 at bark-632.berkeley.edu>,
aspang at mendel.berkeley.edu (Anne Spang) wrote:

> Hi!
> 
> I would like to use brefeldin A in S. cerevisiae. What conc. should be
> used for which cell density? Any help (protocol?) would be appreciated!!
> 
> Thanks in advance!!
> 
>       Anne

I remember hearing that brefeldin A doesn't normally get into yeast cells,
so most strains are resistant. People usually use erg6 mutants which have
a defective plasma membrane that allows some brefeldin A to leak in. I
took a quick look at Biological Abstracts and saw the following
references:

Shah and Klausner (1993) Brefeldin A reversibly inhibits secretion in
Saccharomyces cerevisiae. J Biol Chem 268:5345- 8

Graham, Scott and Emr (1993) Brefeldin A reversibly blocks early but not
late protein transport steps in the yeast secretoy pathway. EMBO J 12:869-
77

Vogel et al. (1993) Brefeldin A causes a defect in secretion in
Saccharomyces cerevisiae. J Biol Chem 268:3040- 3

These papers probably mention what concenrations and conditions to use.
Hope this helps!

-Daniel



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