single colony PCR

Kevin A. Morano kmorano at umich.edu
Tue Sep 10 10:32:09 EST 1996


> Does anybody have a protocol for single colony PCR? (That is, use yeast
> colony directly as PCR template instead of extracted genomic DNA) And how
> well does it work? 


   Check the archives for more detail, but just grow up a fresh plate of
yeast, pick a colony with some sterile implement and swizzle it in your PCR
reaction BEFORE the Taq goes in.  Also helps to include a 10 min precycle
denaturing step (95šC) in your method.
Kevin

-- 
Kevin Morano, Thiele Lab
University of Michigan Medical School
kmorano at umich.edu



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