plasmid isolation from yeast

Kevin A. Morano kmorano at
Wed Sep 11 17:45:38 EST 1996

Someone wanted the buffer recipe for the protocol I mentioned.  Here's the data:

"Rapid isolation of plasmid DNA from yeast",  Current Protocols,
contributed by Charlie Hoffman

Breaking buffer:  2% (v/v) Triton X-100, 1% (v/v) SDS, 100 mM NaCl, 10mm
Tris 8.0, 1 mM EDTA

It's a great protocol!

Kevin Morano, Thiele Lab
University of Michigan Medical School
kmorano at

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