hydroxyurea arrest

Martin Stahl imsd040 at rzbox.uni-wuerzburg.de
Wed Sep 11 16:31:01 EST 1996


Hi everybody!
Can anybody help me with hydroxyurea arrest? I used the amount of
hydroxyurea as published (300 mM), but even the double amount did not
arrest my cells (W303 background) in S-phase.
Even after 5 hours of the arrest I found cells with large and small
buds, as well as unbudded cells. The only effect I could see was that
the fraction of cells with small buds has increased.
Thank you in advance,

Martin Stahl



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