C-terminal epitope tags

DSF 76042.532 at CompuServe.COM
Thu Sep 12 14:35:46 EST 1996


Hi- I have Flag, Flu, myc3, and AU1 c-terminal epitope tags on a 
seven-transmembrane receptor that I've been expressing in yeast. 
Since I didn't have a +ve control for flu, myc and au1, our 
vector man created a mammalian vector that I 
transiently transfected into CHO cells that shows binding in the 
membrane (the transfection worked fine) but there are no specific 
bands on westerns. Any ideas? Any help would be appreciated.
Lynette



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