C-terminal epitope tags

DSF 76042.532 at CompuServe.COM
Thu Sep 12 14:35:46 EST 1996

Hi- I have Flag, Flu, myc3, and AU1 c-terminal epitope tags on a 
seven-transmembrane receptor that I've been expressing in yeast. 
Since I didn't have a +ve control for flu, myc and au1, our 
vector man created a mammalian vector that I 
transiently transfected into CHO cells that shows binding in the 
membrane (the transfection worked fine) but there are no specific 
bands on westerns. Any ideas? Any help would be appreciated.

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