C-terminal epitope tags
76042.532 at CompuServe.COM
Thu Sep 12 14:35:46 EST 1996
Hi- I have Flag, Flu, myc3, and AU1 c-terminal epitope tags on a
seven-transmembrane receptor that I've been expressing in yeast.
Since I didn't have a +ve control for flu, myc and au1, our
vector man created a mammalian vector that I
transiently transfected into CHO cells that shows binding in the
membrane (the transfection worked fine) but there are no specific
bands on westerns. Any ideas? Any help would be appreciated.
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