Tryptophan deficient needed

[Cathy Ball]

Dear Sean May,

Searching the Saccharomyces Genome Database (SGD) shows that the yeast
gene SCM1/TAP2 encodes a tryptophan permease.  Molly Fitzgerald-Hayes and
co-workers have published a paper on this gene (Mol Gen Genet 244: 
260-268 (1994)) and have constructed several mutants.  Try contacting
Molly Fitzgerald-Hayes, Dept. of Biochemistry and Molecular Biology,
Amherst, MA, 01003, USA.  The lab phone number is listed with the SGD as
(413)545-0235. 

Good luck,

Cathy Ball
Saccharomyces Genome Database
Dept. of Genetics, Stanford University
e-mail:  ball at genome.stanford.edu
SGD URL:  http://www-genome.stanford.edu/Saccharomyces/

On Mon, 23 Sep 1996, Dr S T May wrote:

> Hi,
> 
> We are working on a potential tryptophan-like permease in the plant
> Arabidopsis.
> 
> We would like to perform molecular complementation experiments with
> our cDNA, and would therefore like to try transforming tryptophan
> transport deficient yeast mutants.
> 
> We have transformed wild-type yeast and are therefore familiar with
> the techniques, but we are unfamiliar with the availability of
> such mutant strains and would like to hear from anyone who might
> be able to point us in the right direction.
> 
> Thanks for any help.
> 
> Sean May / Joanna Kargul
> 
> 
> 
> -------------------------------------------------
> Sean May  [sean.may at warwick.ac.uk]
> Warwick University, Coventry, U.K.
> HTTP://www.oikos.warwick.ac.uk/~bioys/index.html
>