1 kb DNA Ladder

kleinh01 at mcrcr6.med.nyu.edu kleinh01 at mcrcr6.med.nyu.edu
Tue Sep 24 07:34:36 EST 1996

In article <Pine.A32.3.91.960923174617.83656A-100000 at spider.uspnet.usp.br>, scamargo at usp.br (Solange S de Camargo) writes:
> 	Does anybody know why the 1 kb DNA Ladder hybridizes with an 
> yeast DNA probe? 
> 	I read in the GIBCO BRL  catalogue that this molecular size 
> contains vector DNA fragments. I looked for more details but I didn't 
> found. Does anybody know which is the "vector" they used to construct 
> this marker?? I had three beautiful bands after hybridizations experiments 
> using LEU2 gene like a probe (1,636; 506 and 396 pb). Have already 
> happened the same with someone?  
> 	Solange

Those bands contain some pBR322 DNA.  Your probe is a little dirty. 
The remaining bands are from yeast 2 micron fragments.  All of this is
in the GIBCO BRL biospecs on the 1 kb ladder.

Hannah Klein
kleinh01 at mcrcr.med.nyu.edu

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