Glycerol in Freezing media

Achim Recktenwald, PhD achim at ibex.ca
Wed Sep 25 07:35:00 EST 1996


Louis Geller wrote:
> 
> Hi.
>         I was wondering exactly it is necessary to have glycerol in freezing
> medium for bacteria, yeast, and animal cells.
>         Exactly how does the glycerol protect the cells?
>         Thanks in advance,
>         Louis Geller...
>         lgeller at csulb.edu
>         bbbbd at sprynet.com

It either prevents - or reduces at least - the production of sharp edged
water crystals that could pierce the cell walls.

Achim



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