Glycerol in Freezing media

rand777 at ids.net rand777 at ids.net
Fri Sep 27 03:27:57 EST 1996


In Article <52eqej$3gh at solaris.cc.vt.edu>
dna at vt.edu (dna) writes:
>In article <3248D5D3.4F9A at sprynet.com>, bbbbd at sprynet.com says...
>>
>>Hi.
>>        I was wondering exactly it is necessary to have glycerol in 
>freezing
>>medium for bacteria, yeast, and animal cells.
>
> Yes it is.
>Try a little experiment where you add no glyc. 10%, 20%, 30% ...
>Freeze them at - 80C overnight.
>Then plate 50ul on non-selective media.
>This will show you the results.
>
>The Glycerol supposedly keeps ice crystals to a minnimum which allows the 
>cells to survive.
>
>-- 
>John R. McQuiston
>Molecular Genetics Lab
>CMMID VMRCVM
>VPI & SU
>1410 Prices Fork Rd
>Blacksburg VA 24061
>jmcquist at vt.edu
>
=====================================================================

Viability of frozen organisms is very much determined by the medthod of
thawing. For maximum survial, one should place the container of frozen 
organisms in a *cold* waterbath for thawing. A quick thaw will result
in very low survial rates.  R.Randolph-rand777 at ids.net




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