Required culture volume for protein isolation

Louis Geller bbbbd at sprynet.com
Mon Sep 30 02:18:46 EST 1996


Hi.
	A colleague of mine is attempting to isolate a protein from
Cryptococcus neoformans.  He is unsure what volume of culture should be
grown up, prior to cell harvesting and lysis.  Also, what OD600 or
density should he grow the cells to?
	I don't think it is an abundant protein.
	Thanks for any help or references you can provide.
	Please respond here to via email to lgeller at csulb.edu.

	Thanks,
	Louis Geller
	Graduate Student in Cell & Molecular Biology - CSU Long Beach



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