Pichia pastoris

Colin Loney c.loney at scorch.vir.gla.ac.uk
Wed Apr 2 03:13:15 EST 1997


Is anybody using the Pichia expression system from Invitrogen? If so
are you encountering any problems digesting the pPIC3.5 or pHILD2
vectors supplied with the system?  I am using standard methodology but
have so far been unable to digest these vectors to completion or
ligate a 1kb fragment into them. Any ideas would be welcome. Thanks in
advance .
MRC Virology Unit
Institute of Virology
Glasgow



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