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Yeast nucleus staining

Kevin A. Morano kmorano at umich.edu
Thu Apr 3 17:38:28 EST 1997

1)  Fix cells for 30 minutes in 70% ethanol.  
2) Resuspend in 1  ml  0.1% Nonidet P-40, 10 mM NaCl, 50 mM sodium citrate
pH 7.0.  Add Pi to 100 ug/ml.  Incubate on benchtop for 30 min.
3) Pellet and wash cells twice, resuspend in 50 mM citrate buffer .
4) View under microscope, stable for at least a few hours.  VERY intense.

Try this.  Cells are dead of course, so this only works for terminal
experiments.  It's essentially a stripped-down flow cytometry protocol.


Kevin A. Morano
Thiele Lab                                                 
Dept. of Biological Chemistry
University of Michigan Medical School
Ann Arbor, MI 48109-0606
kmorano at umich.edu

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