GFP help please

Todd Hryciw toddh at duke.usask.ca
Tue Apr 15 14:26:29 EST 1997


Hi,
I'm trying to localize a protein (roughly) using a GFP tag, and was
wondering if the tag is supposed to fluoresce under UV light.  I can see
greenish-yellow things under the fluorescent light and when I switch to
the
UV filter, they are still there.  These yellowish dots are present only at
a low frequency in my vector control.
Thanks for your help.
Todd Hryciw
Dept. of Microbiology
U. of Saskatchewan
Canada 




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