Fixation of GFP-fusion cells

Mark Flory mflory at u.washington.edu
Thu Apr 17 19:57:07 EST 1997


Jae,

In S.cerevisiae, we have found that a 15 minute formaldehyde (3.7%) 
fixation on ice maintains the GFP signal when using the S656T GFP 
derivative.  This may or may not work in pombe (haven't tried).  Good 
luck!

Mark Flory
Dept. of Biochemistry
University of Washington
Seattle





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