ura3 deletion

Chuck Staben staben at pop.uky.edu
Mon Apr 21 16:17:19 EST 1997


I would like to use ura3 to do a PCR-knockout.  The ura3-52 allele is
not appropriate since it is a Ty-1 insertion, not a deletion.  Does
anyone have an appropriate ura3 deletion, analagous to the
his3-delta200?

Secondly, where does one look in a systematic way for such strains other
than YGSC?




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