electroporation of S. pombe

Nick nick_owen at wo*ls.demon.co.uk
Sun Aug 31 04:09:17 EST 1997


On 27 Aug 1997 07:48:12 -0700, amerla at ZOO.UVM.EDU wrote:

>Does anyone have a good protocol for electroporating S. pombe?
>I'm going to be doing a *lot* of pombe transformations soon and
>could use a protocol that is more rapid than the standard lithium 
>acetate protocol and has comparable to higher levels of transformants.
>
>Thanks!
>
>Alia


I have been using the electroporatioon method on the Forsburg web site
for a number of months now and it works very well , both haploids and
diploids

Visit:

http://flosun.salk.edu/users/forsburg/tfmn.html

Nick

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