EMS mutagenesis protocol/tips?

Erickson edumaual at csulb.edu
Wed Dec 10 16:39:28 EST 1997


I am going to construct a kill curve (~50%) on S. cerivisiae with use
of EMS.  I a'm wondering if anyone has a protocol or advice they can
offer.  I have never attempted mutagenesis with EMS and would like a
rough idea of what concentration I should use as a starting point.


Thanks

Erickson <edumaual at csulb.edu>






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